Scheduled System Maintenance:
Some services will be unavailable Sunday, March 29th through Monday, March 30th. We apologize for the inconvenience.
By Topic

Application of SELDI technique: Salivary protein fingerprint spectrum in the diagnosis of thyroid cancer

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

The purchase and pricing options are temporarily unavailable. Please try again later.
4 Author(s)
Sun Kehuan ; Second Clinical Med. Coll., Ji''nan Univ., Shenzhen, China ; Cao Meiqun ; Wu Zhengzhi ; Li Lujun

Objective: Using surface-enhanced laser desorption/ionization (SELDI) mass spectrometry to test the salivary protein fingerprint from the patients with thyroid cancer, benign nodular goiter and healthy subjects, to screen out the differentially expressed proteins between each group, and establish a diagnostic model with the help of bioinformatics to provide a novel approach for the early diagnosis of thyroid cancer. Methods: Saliva protein fingerprints of 49 patients with thyroid cancer, 34 patients with nodular goiter and 43 healthy subjects were tested using SEDLI technique. Specific differentially expressed proteins in the saliva of thyroid cancer patient were screened out and a diagnostic model was established with the help of bioinformatics. Results: The total accuracy of the cross validation (test group) for the diagnostic model of thyroid cancer and health control was 81.8% (72/88), sensitivity 88.9% (40/45), specificity 74.4% (32/43); The total accuracy of the cross validation (test group) for the diagnostic model of thyroid cancer and nodular goiter was 89.1% (74/83), sensitivity 87.7%(43/49), specificity 91.1% (31/34). Conclusions: By using SELDI technique, a preliminary diagnostic model for thyroid cancer was established, which may provide a novel approach of high sensitivity and strong specificity for the early diagnosis and pre-operation diagnosis of thyroid cancer. It deserves further investigation and application.

Published in:

Information Technology in Medicine and Education (ITME), 2012 International Symposium on  (Volume:2 )

Date of Conference:

3-5 Aug. 2012