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In recent years, there has been a dramatic proliferation of research concerned with heart rate variability (HRV) analysis. Heart rate variability (HRV) analysis is a largely used method for accessing cardiac autonomic control. Based on HRV, the standard deviation of normal-to-normal (SDNN) heart beats and the ratio of low frequency (LF) and high frequency (HF) power of RR intervals can be used for monitoring the activity of circulation system and the sympatho-vagal balance of autonomic nervous system (ANS) respectively. The purpose of the research presented in this paper is to examine the relationship between tempo and complexity of music and the two variables of the HRV measures. A four-phase study was designed to take down the values of the two variables. Subjects were 26 students enrolled in the National Chiao Tung University and were tested individually in the laboratory. In this investigation we recorded four different phasis values of the two variables. The results revealed a medium level of consistency between the two variables and the very fast and complex style rhythm. Clearly, the findings indicate the very fast and complex style rhythm has a positive effect on HRV. To conclude, these findings lead us to believe that very fast and complex style rhythm should be used to treat the symptom of people who cannot concentrate on doing something such as suffering from melancholia.