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In this study, we have developed a prototype of wireless ECG biofeedback platform which consists of three parts: a full-featured signal acquisition platform, a full set of software applications for signal displaying, processing and analysis, and a matlab-based audio generator. Moreover, preliminary experiments on heart rate variability (HRV) have been performed to evaluate the performances of this platform and investigate the changes of HRV in cognitive unconscious status. 23 volunteers were recruited in this experiment, time domain, frequency domain and geometrical parameters of HRV were chosen to analyze in this study. All the results provided a physiological evidence for Positive Psychotherapy theory (PPT) and also demonstrated that this prototype platform was effective for HRV-based biofeedback which may enable emotion self-regulatory and management in daily life.