By Topic

The role of GSH depletion in Resveratrol induced HeLa cell apoptosis

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Bo Zhang ; Sch. of Pharm. Sci., Univ. of Shihezi, Shihezi, China ; Xiao-qin Wang ; Hanying Chen ; Qiusheng Zheng
more authors

The dual role of Resveratrol (Rsv) in cell apoptosis was recently reported by its anti/pro-oxidant activities. The involvement of ROS and GSH was thus investigated in Rsv-induced HeLa cell apoptosis. Rsv, higher than 10 μM, elevated the intracellular ROS but reduced O2- and GSH levels. ROS scavengers (Tempol, catalase) could not inhibit the apoptosis. Treatment with GSH modulators DTT or BSO were resulted up-regulation or down-regulation GSH levels, but both enhanced Rsv-induced HeLa cell apoptosis. However, BSO could not prevent the DTT+Rsv treated HeLa cells from apoptosis. Further, Rsv-induced HeLa cell apoptosis was accompanied by activation of caspase 3 but not caspase 9, neither did the loss of mitochondrial membrane potential. Conclusively, the changes of ROS by Rsv were not tightly correlated with apoptosis in HeLa cells. However, intracellular GSH levels are partially related to Rsv-induced HeLa cell apoptosis via a mitochondrial independent manner.

Published in:

Systems Biology (ISB), 2011 IEEE International Conference on

Date of Conference:

2-4 Sept. 2011