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This paper presents an assessment of current natural hydro-biological resources in Lijiang River, Guangxi, China. Previous investigations and evaluations in 1974-1976 and 1980-1982 were compared to determine possible improvement in aquatic ecosystem of the river. The assessment results under current condition of the river demonstrated that natural hydro-biological resources and the diversity of the ecosystem of the main channel of the river were not well-preserved. The river's environmental assimilation capacity was becoming vulnerable. The symptom of natural resources exhaustion and decline in fish was also found, which indicates that genetic and species diversity in the river is still endangered.