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Sensitivity Enhancement of Leakage Current Microsensor for Detection of Target Protein by Using Protein Denaturant

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6 Author(s)
Noda, M. ; Grad. Sch. of Sci. & Technol., Kyoto Inst. of Technol., Kyoto, Japan ; Lorchirachoonkul, P. ; Shimanouchi, T. ; Yamashita, Kaoru
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We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K 4[Fe(CN)6] solution to detect the existence of proteins. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully resulted in a prominent improvement in sensitivity by 129-folds of magnitude. This is because the modulation of conformation of the target proteins, carbonic anhydrase from bovine (CAB) and lysozyme could contribute to the promotion of the interaction between the liposome and the target protein.

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Sensors Journal, IEEE  (Volume:11 ,  Issue: 11 )