Open-access phototransistor-based optoelectronic tweezers for long-term single cell heterogeneity study

We demonstrate, for the first-time, long-term culturing of single cells under continuous phototransistor-based optoelectronic tweezers (Ph-OET) trapping. A new device configuration, open-access Ph-OET, is presented to integrate the trapping functionality of Ph-OET with culturing capabilities in a Petri dish. Maintenance of human leukemia cell lines (K562, Jurkat) under optical manipulation for up to five cell generation has been achieved.