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Mental workload is useful for evaluating performance of a ship's navigator: a captain, a duty officer, and a pilot. The heart rate variability (R-R interval), the nasal temperature and the salivary amylase predict well based on pre-experiments; however, most of the research tests a professional's skill. The evaluation does not test a cadet's skill yet. In this paper, we evaluate a cadet's R-R interval as they guide a ship from a narrow channel to open sea, and consider simulator training effects. The experiment is carried out using a ship bridge simulator, not a real ship. We show the R-R interval is a good index for the evaluation of ship bridge teamwork for marine simulator training.