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This paper presents the procedure of determination of the quantum efficiency of luminescence of Mn2+ ions in the Zn1-x-yBexMnySe crystals. The method is based on the photoacoustic spectroscopy approach. In the paper the experimental spectra of absorbance, transmission, absorption and photoacoustic spectra of the samples are presented and analyzed from the point of view of the possibility of determination of the quantum efficiency of Mn2+ ion luminescence at room temperature. It was determined experimentally that in the investigated crystals the quantum efficiency of luminescence in the Mn2+ ions is about 35%, 40%, 32% for the absorption peaks at 430 nm, 470 nm and 510 nm respectively for Zn0.75Be0.2Mn0.05Se crystal.