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In RFID system, more than one tag answering the query of reader together can result in tag collision, which makes the communication between reader and tag failure. For the problem, a novel anti-collision algorithm was present in the paper. The new algorithm transmitted the maximal collision bit position but the serial number of tag, which reduced the data transmitted greatly, and uses splitting to make the algorithm working in a smaller scope which reduces the number of searching. Computer simulation result shown that the new algorithm was efficient.