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In this work, styrene butadiene rubber (SBR) nanocomposites were produced at three (5, 10, 15 phr) different levels of loading by using after hatching eggshells (AHES) as bio-fillers and their thermo-mechanical properties were well compared with those prepared by calcium carbonate (CA) nanofillers. AHES fillers were first surface modified by stearic acid and consequently were used while CA nanofillers were coated by provider. Tensile strength and elongation at break data showed considerable improvement through employing AHES nanofillers. In addition, thermogravimetric analysis onto produced samples showed higher thermal stability of samples which were fed with AHES at 5 phr loading than that of CA nanofillers and similar thermal stability of nanocomposites were obtained when 10 and 15 phr of both kinds of nanofillers were used separately.