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In this paper, it demonstrates a CMOS-compatible transducer for creatinine detection. Different with traditional application of ISFET (Ion Selective Field Effect Transistor), it is not for the hydrogen ion detection. To combine the CMOS MEMS platform with MIP (Molecular Imprinting Polymer) micro coating technologies, the developed MlP-gate ISFET could be used for the detecting of creatinine. The experiment results showed that the difference of response current was about 13? A when the concentration was varied from 0.199 mg/mL to 0.787 mg/mL. Beside, this paper successfully demonstrates the possibility of the liquid isolation and localized MIP coating technology.