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Environmental test is widely used to verify the adaptability and reliability of various electronic products in the last decades. The sharp change of temperature and humidity in certain situations could pose a serious reliability issue on the electronic components. This paper presents an experimental study of one kind of plastic substrate based flexible display called STN (super-twisted nematic). The study focuses on the reliability of display under various temperature and humidity conditions. Considering the range of temperature and humidity changed in applications, the experiments are divided into four subgroups: cold test, cyclic damp heat test, the steady state damp heat test and dry heat test. Through all the tests, the STN display reliability was evaluated and the failure modes were obtained. Based on these tests, the thermal-structural simulation model was built. By the simulation, part of the experiment phenomenon were explained. It provides a solid foundation for further reliability study of the STN display in the practical applications.