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Effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice

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4 Author(s)
Zou Xiang ; Minist. of Educ., Eng. Res. Center of Natural Anticancer Drugs, Harbin ; Ji Yu bin ; Ji Chen Feng ; Xiao Feng

To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice a tumor mouse model was developed, and the mice were treated with high, medium, and low doses of qinglongyi polysaccharide through abdominal injection for 7 d. Erythrocytes were collected and erythrocyte suspensions were prepared. Change of Ca2+ concentration in the erythrocytes of S180 mice was measured by stained with Fluo-3/AM and observed with laser scanning confocal microscope, Change of H+ concentrations measured by stained with BCECF-AM and observed with laser scanning confocal microscope. Results showed Qinglongyi polysaccharide lowered the concentration of Ca2+ and H+ in erythrocytes of S180 mice, which is remarkable compared with negative control group(PLt0.05). Conclusion Qinglongyi polysaccharide can maintain the stability and balance of cation concentration in erythrocyte of S180 mice.

Published in:

Complex Medical Engineering, 2009. CME. ICME International Conference on

Date of Conference:

9-11 April 2009