By Topic

Study on HepG-2 apoptosis induced by saponins isolated from asparagus and the effects on the activities of Caspase-3,8,9

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Yu bin Ji ; Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, China ; Chen feng Ji ; Lei Yue ; He Xu

To study the effect of saponins of asparagus on apoptosis and the variations of Caspase-8, 9, 3 activity in the process of asparagus induced apoptosis in HepG-2. Cell was administrated with different concentrations of asparagus at different time, the apoptosis rate was determined by FCM with AV-PI staining, the morphology was observed by electron microscopy, caspase-8,9,3 activities was measured by kit. Experiments showed that saponins of asparagus inhibited cell growth. At 72 h, the apoptosis rate increased with concentrations markedly. Apoptotic morphology was observed. After Asparagus officinalis saponin of different concentrations have been acting on HepG-2 cells for 24 h and 48 h, the activity of caspase-8, caspase-9, and caspase-3 was significantly increased compared with that in the negative control, which suggesting that the induction of apoptosis in tumor cells by Asparagus officinalis saponin may be related to its activating caspase-8, caspase-9, and caspase-3. From the experimental results, it can also be seen that the activity of Caspase-8 is high at 24 h, and the activities of Caspase-9 and Caspase-3 is high at 48 h, which suggesting that, in inducing apoptosis in HepG-2 cells, Asparagus officinalis saponin first activates the starter caspases, or caspase-8 and caspase-9, and the activation of caspase-8 and caspase-9 in turn leads to the caspase cascade, resulting in the activation of caspase-3. As an executor caspase, caspase-3 eventually leads to cell apoptosis.

Published in:

Complex Medical Engineering, 2009. CME. ICME International Conference on

Date of Conference:

9-11 April 2009