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Microfluidic formation of lipid bilayer array for membrane transport analysis

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4 Author(s)
Sadao Ota ; Center for International Research on Micromechatoronics, Institute of Industrial Science, The University of Tokyo, Japan ; Wei-Heong Tan ; Hiroaki Suzuki ; Shoji Takeuchi

We present a highly parallel and reproducible method for reconstituting an array of lipid bilayers to analyze membrane transport. We infuse buffer/lipid/buffer solutions sequentially into a microchannel with numerous microchambers in its walls and seal each chamber by a lipid bilayer containing membrane proteins. Due to the small volume of the chamber (2 pL), membrane transport of confined fluorescent molecules across the bilayer through the proteins is readily observed as changes in fluorescent intensity. We successfully perform quantitative measurement of the transport flux of fluorescent molecules (calcein) through alpha-hemolysin antibiotic pores.

Published in:

Micro Electro Mechanical Systems, 2008. MEMS 2008. IEEE 21st International Conference on

Date of Conference:

13-17 Jan. 2008