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A stochastic model for the efficiency of polymerase chain reaction (PCR) processes is presented. The model is based on the assumption that the i'th nucleotide incorporation on the DNA template takes taui seconds, where taui has an exponential distribution with mean 1/lambdai. In this paper, given lambdai that can be obtained from the primer-template sequence, temperature profile, enzyme rate, and other assay conditions, we calculate the efficiency of a multi-step PCR process using the distribution of the summation of non-identical exponential distributions.
Date of Conference: 28-30 May 2006