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A foil-based BioMEMS for electrochemical capillary immunoassay for hepatitis serology is presented. Immobilization of the hepatitis antigen utilizing capillary forces and special stopping barriers in foil based microsystems and the application of it in fast immunoassays will be shown. The measurement starts with the addition of the antibody containing human serum sample. Subsequently the signal antibody labelled with glucose oxidase binds to the hepatitis B antibody and converts glucose to hydrogen peroxide, which is oxidized at the electrode in the measurement cell. The assay is very sensitive and designed to differentiate between hepatitis B negative (below 10 U/l) and positive serum. The present limit of detection of immunoassays in the BioMEMS is 20 atto-moles.
Date of Conference: 5-9 June 2005