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The research disclosed a particle-counting device which can reliably count particles in the fixed quantity of sample solution. Let sample drop in the chip inlet, then use dielectrophoresis (DEP) force produced by separation device on the chip to separate and move specific particles to micro-pore. High reliable Coulter principle had been introduced on this device. When the particles pass through the micro-pore one by one, recording the numbers of ion current reduction events can correctly count the quantity of specific particles in the fixed volume of sample solution.