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In this paper, the split and recombination (SAR) micromixer is fabricated using microfabrication process for PDMS and evaluated efficiency of SAR mixing method which increase interfaces exponentially. Simulation using CFD-ACE+ shows a cross-sectional view of flow and estimates mixing efficiency of the SAR micromixer. Mixing experiment of phenolphthalein and NaOH solution shows interfaces as red lines increased by SAR mixing. The result of mixing experiment of blue dye and water is evaluated mixing efficiency by calculation of standard deviation of pixel intensity of the observed image. After 7th unit of the SAR micromixer, fluids are mixed 90% at Re 0.6099. More units are demanded for complete mixing when flow rate increases.