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A fiber-optic sensor sensitive to hydrogen peroxide has been designed based on the electrostatic layer-by-layer self-assembly method. Meldola's blue and a catalyst hemin have been deposited in a polymeric structure formed by PAH+ and PAA-. The concentrations that can be detected range at least between 10-7 and 10-1 M, and recovery of the sensor after introduction in a reductive agent has been proved successfully. Some rules for estimation of the refractive index of the material deposited and the thickness of bilayers are also given.