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3-D reconstruction of 2-D crystals in real space

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6 Author(s)
Marabini, R. ; Escuela Politecnica Superior, Univ. Autonoma de Madrid, Spain ; Sorzano, C.O.S. ; Matej, S. ; Fernandez, J.J.
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A new algorithm for three-dimensional reconstruction of two-dimensional crystals from projections is presented, and its applicability to biological macromolecules imaged using transmission electron microscopy (TEM) is investigated. Its main departures from the traditional approach is that it works in real space, rather than in Fourier space, and it is iterative. This has the advantage of making it convenient to introduce additional constraints (such as the support of the function to be reconstructed, which may be known from alternative measurements) and has the potential of more accurately modeling the TEM image formation process. Phantom experiments indicate the superiority of the new approach even without the introduction of constraints in addition to the projection data.

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Image Processing, IEEE Transactions on  (Volume:13 ,  Issue: 4 )