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A new input function monitoring system has been developed using a GSO detector for both PET and SPECT quantitative studies. The paired assembly of crystals provided an absolute sensitivity of approximately 7% for PET tracers and 70% for 99mTc and 201Tl (SPECT tracers). This system was applied to clinical use and animal study such as monkey and rat. This study demonstrates that the present system can he of use in both clinical and small animal studies using SPECT and PET tracers.