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The paper shows an application of a new operator named the iterated object transform (IOT) for cell classification. The IOT has the ability to grasp the internal structure of a digital object and this feature can be usefully applied to discriminate structured images. This is the case of cells representing chondrocytes in bone tissue, giarda protozoan, and myeloid leukaemia. A tree classifier allows us to discriminate the three classes with a good accuracy.