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The development of a computer-controlled piezomanipulation system is presented for biomedical applications involving cells manipulation. An application of the developed system to intracytoplasmic sperm injection (ICSI) is presented. The hardware setup and the control strategies are described in full detail to illustrate the advantages of this approach compared to manual injection methods. The system developed offers the automated and efficient execution of the ICSI process, thus achieving an improved success rate in terms of the end objectives of the process.