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A new technique based on freezing curve monitoring to quickly evaluate the viability of the biological materials subject to freezing or rewarming was established. A practical integrated device was fabricated which is simple in structure and cheap in price. Preliminary freezing experiments on the fresh fish blood demonstrated that minor changes in a biological material due to freezing or warming injury may result in significant deviation in its freezing curve compared with that of the intact biomaterials. Three thermal indexes to quantify the damage degree of the biomaterials were pointed out. This method also opens many opportunities for the evaluation of biological material defect in diverse life science fields.