By Topic

Imaging unfixed cell membranes by atomic force microscopy

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Popescu, A.L. ; Lab. of Biophys. Eng., Pennsylvania Univ., Philadelphia, PA, USA ; Law, R.J. ; Discher, D.E.

Due to their softness, cell membranes are difficult to imagine by AFM without chemical fixation or drying. Using the simple erythrocyte we present a procedure of tensing the erythrocyte membrane by spreading and immobilizing cells on a poly-L-lysine layer. This spreading tenses the upper membrane and generates a spherical cap, facilitating AFM imaging of the unfixed erythrocytes. Images were obtained in tapping mode at low scan rates avoiding damage of the membrane and proving highly reproducible. On both red cells and ghost cells indentation could be made deep enough to reach the steric core of the two membranes (upper and lower) giving a thickness of 42±15 nm (126 cells) under a maximum compression force of 2 nN. Detailed images of the erythrocyte membrane show stable corrugated structures about 7 nm in height, suggesting indentation of the membrane lipid bilayer down to the underlying cytoskeletal network

Published in:

Bioengineering Conference, 2002. Proceedings of the IEEE 28th Annual Northeast

Date of Conference:

2002