By Topic

Characterization of neuronal cell injury and neuroprotective effect of Poloxamer

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Serbest, G. ; Sch. of Biomed. Eng., Sci., & Health Syst., Drexel Univ., Philadelphia, PA, USA ; Horwitz, J. ; Barbee, K.A.

To better understand the cellular mechanisms of neuronal injury, it is very important to mimic mechanical loading conditions experienced by the cells in vitro. Mechanical force applied to cells outside may activate some downstream events inside the cells, which may further cause necrosis or apoptosis. In this study, we used a cell culture model, which is an idealized system to investigate cell injury at the cellular and molecular levels. We developed an injury model, which allows a precisely controlled mechanical stimuli and an easy quantification of cellular responses. A dynamically controlled shear stress was applied on PC2 cells and cell viability was assessed at 24 hours. Dynamic mechanical loading of cells produced graded levels of injury as assessed by long-term viability. We also used Poloxamer to demonstrate its neuroprotective effect on the injured cells. This system can be used to investigate further the mechanism of the injury and to assess various treatments of neuronal injury and subsequent degeneration

Published in:

Bioengineering Conference, 2002. Proceedings of the IEEE 28th Annual Northeast

Date of Conference:

2002