By Topic

Automated, integrated modules for fluid handling, thermal cycling and purification of DNA samples for high throughput sequencing and analysis

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

12 Author(s)
Meldrum, D.R. ; Dept. of Electr. Eng., Washington Univ., Seattle, WA, USA ; Pence, W.H. ; Moody, S.E. ; Cunningham, D.L.
more authors

The Genomation Laboratory at the University of Washington has developed an automated fluid handling system called “Acapella” to prepare microliter reactions for genome analysis. Reactions such as restriction enzyme digests, polymerase chain reactions (PCRs), and sequencing reactions are prepared in glass capillaries, one per sample, with an automated system that can process 5000 samples in 8 hours. A DNA sample is aspirated from a microplate well with a novel piezoelectric aspirator/mixer actuator, reagents are dispensed into the end of the capillary with piezoelectric reagent dispensers, all fluids inside the capillary are mixed with the piezoelectric aspirator/mixer actuator, and the capillary is off-loaded to a capillary cassette for further processing. On-going development includes new, automated modules for thermal processing of capillaries, real-time DNA quantitation, and purification of DNA inside of capillaries to prepare the samples for DNA sequencing. Applications of the technology include minimal residual disease quantification and sample preparation for DNA sequencing

Published in:

Advanced Intelligent Mechatronics, 2001. Proceedings. 2001 IEEE/ASME International Conference on  (Volume:2 )

Date of Conference:

2001