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3-D multiphoton laser scanning confocal microscopy as a probe for subsurface defects in polymers

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8 Author(s)
Bhawalkar, J.D. ; Dept. of Chem., State Univ. of New York, Buffalo, NY, USA ; Swiatkiewicz, J.J. ; He, G.S. ; Prasad, P.N.
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Summary form only given. Confocal laser scanning microscopy (CLSM) provides a significant improvement in axial resolution over conventional epi-fluorescence microscopy by eliminating out-of-focus fluorescence. This is achieved by using a spatial filter in the form of a confocal aperture. The use of IR leads to a larger penetration depth in materials, providing an opportunity to image thicker samples. Furthermore, it is possible to open up the entire visible spectrum for multiple detection channels. The fluorophore, 4-[N-(2-hydroxyethyl-N-methyl) amino phenyl]-4'-(6-hydroxyhexyl sulfonyl)stilbene (APSS), exhibits a strong two-photon absorption at 800 nm, and green upconverted fluorescence emission.

Published in:

Lasers and Electro-Optics, 1996. CLEO '96., Summaries of papers presented at the Conference on

Date of Conference:

2-7 June 1996

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