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Ab initio reconstruction and experimental design for cryo electron microscopy

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2 Author(s)
Doerschuk, P.C. ; Sch. of Electr. & Comput. Eng., Purdue Univ., West Lafayette, IN, USA ; Johnson, J.E.

A statistical model for the object and the complete image formation process in the cryo electron microscopy of viruses is presented. Using this model, maximum-likelihood reconstructions of the three-dimensional (3-D) structure of viruses are computed using the expectation maximization algorithm, and alternative experimental designs are evaluated based on Cramer-Rao bounds. Numerical examples of the reconstructions and experimental design evaluations are provided based on Cowpea mosaic virus

Published in:

Information Theory, IEEE Transactions on  (Volume:46 ,  Issue: 5 )

Date of Publication:

Aug 2000

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