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Migration and adhesion of lymphocytes cultured under low shear conditions

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2 Author(s)
Bergman, A.J. ; Dept. of Chem. Eng., Rice Univ., Houston, TX, USA ; Zygourakis, K.

Mechanical forces have an important effect on lymphocyte migration and adhesion. Culturing Jurkat cells (a T lymphoblastoid cell line) in a low-shear environment has a profound effect on the migratory behavior of the cells, but no significant effect on their adhesion strength to fibronectin-coated surfaces. Using a 2-D cell migration assay, the authors determined that cells cultured in a low shear reactor system for 4-10 days had a significantly higher cell speed than cells cultured statically. Analysis of cell trajectories using the persistent random walk also revealed a higher RMS cell speed and random motility coefficient for cells cultured in the low shear reactor. One possible explanation for these differences is an increased integrin alpha-5 subunit expression on cells cultured in the low shear environment

Published in:

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint  (Volume:2 )

Date of Conference:

Oct 1999