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Oriented assembly of protein on surfaces

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3 Author(s)
Bachyas, L.G. ; Dept. of Chem., Kentucky Univ., Lexington, KY, USA ; Bhattacharyya, D. ; Anderson, K.W.

Several approaches that facilitate the oriented assembly of proteins on a variety of surfaces, including membranes, is described. Biotinylation reactions performed under controlled conditions were used to immobilize enzymes in a layer-by-layer fashion onto surfaces modified with avidin or streptavidin. For other enzymes, a specific attachment site was introduced by gene fusion or site-directed mutagenesis. These strategies were applied to achieve site-specific immobilization of several enzymes including alkaline phosphatase, organophosphorus hydrolase, horseradish peroxidase, and subtilisin. The site-specific immobilization led to orientation of the enzyme molecules on the surface of materials and to a higher activity compared to conventional immobilization methods

Published in:

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint  (Volume:2 )

Date of Conference:

Oct 1999