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Innovations in two-photon deep tissue microscopy

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5 Author(s)
Buehler, C. ; Dept. of Mech. Eng., MIT, Cambridge, MA, USA ; Kim, Ki Hean ; Dong, C.Y. ; Masters, B.R.
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The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent spectroscopic measurements in deep tissue.

Published in:

Engineering in Medicine and Biology Magazine, IEEE  (Volume:18 ,  Issue: 5 )

Date of Publication:

Sept.-Oct. 1999

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