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Using BODIPY dye-primer chemistry in large-scale sequencing

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7 Author(s)
Muzny, D.M. ; Baylor Coll. of Med., Houston, TX, USA ; Metzker, M.L. ; Bouck, J. ; Gorrell, J.H.
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The issues related to integration of BODIPY dyes for large-scale sequencing, as well as analysis of their performance in our large-scale sequence assemblies, are discussed. Topics covered include: storage of BODIPY dye-primers, development of reliable reaction conditions, development of preformatted BODIPY dye-primer cocktails, development and testing of robotics applications, and quality assessment of BODIPY dye-primer reactions in production sequencing.

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Engineering in Medicine and Biology Magazine, IEEE  (Volume:17 ,  Issue: 6 )