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Low resolution 3D reconstructions of viruses from X-ray crystal diffraction data

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4 Author(s)
Yibin Zheng ; Sch. of Electr. & Comput. Eng., Purdue Univ., West Lafayette, IN, USA ; Tao Li ; P. C. Doerschuk ; J. E. Johnson

We describe low-resolution 3D reconstructions of viral particles based on low-resolution (e.g., 20 Å) X-ray crystal diffraction data. The reconstructions are based on parameterized models because the number of data points is small, e.g., 3×102. Reconstruction is then equivalent to parameter estimation which we perform via nonlinear least squares. The focus is on the structure of the nucleic acid and we demonstrate that inclusion of models for the nucleic acid can reduce the R factor and that the resulting parameter values are in accord with simple physiochemical measurements

Published in:

Image Processing, 1998. ICIP 98. Proceedings. 1998 International Conference on

Date of Conference:

4-7 Oct 1998