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In the agricultural production, recently there are some problems in the determination of the amount of soil nitrate nitrogen using ion selective electrode such as low accuracy and long detection time. The research on measurement model of soil nitrate nitrogen based on ion selective electrode is carried out in this article aiming to obtain the information of soil nitrate nitrogen quickly and accurately. Simple linear regression equation is adopted to establish the calibration equation of ion selective electrode and the calculated values of this model compared with laboratory measurements show that the model is quite accurate. Then "stepwise fitting method" is used to establish the three-dimensional mathematical model which also has wide application values among the concentration of test solution, the measuring potential of ion selective electrode and the temperature of test solution.