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We propose and fabricate a novel temperature sensor based on the optical trapping technology. The temperature sensing cell is constructed by putting a “test-micro-particle” enclosed in a space built by a quartz capillary tube and two opposite-inserted optical fibers. In order to make the temperature sensor have the ability of auto-ready and easy-reset, we design and fabricate the special concavities in the ends of two fibers. This ability of auto-ready and easy-reset makes the sensor convenient to be applied in industrial fields for long-term-use. These properties provide a new probably development direction in sensing research fields for the optical tweezers technology, and solve the optical tweezers measurement repeatability problems.