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In this work the results of electrohydrodynamic (EHD) secondary flow measurements in a spike-plate type electrostatic precipitator (ESP) are presented. In the investigated ESP two-sided and one-sided spike electrodes were used as a discharge electrodes. The results of 2D PIV measurements showed that flow pattern obtained for a different spike tips positions in respect to the primary flow direction significantly changing the flow structures in the ESP duct. The submicron dust particles collection measurements in this ESP were also performed. The obtained results showed that the collection efficiency of submicron dust particles depends on the generated EHD secondary flow.