Cart (Loading....) | Create Account
Close category search window
 

System for the Nanoporation of Biological Cells Based on an Optically-Triggered High-Voltage Spark-Gap Switch

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

9 Author(s)
Balevicius, S. ; Center for Phys. Sci. & Technol., Vilnius, Lithuania ; Stankevic, V. ; Zurauskiene, N. ; Shatkovskis, E.
more authors

This nanosecond electric pulse generator is designed for the electroporation of biological cells suspended in a liquid media. It is based on a spark-gap switch, which is optically triggered by a 0.45-ns duration and 1-mJ energy laser pulse (wavelength 1062 nm). This system can also be triggered manually by changing the distance between the spark-gap electrodes. It is able to generate in a 75- Ω impedance transmission line near-perfect square-shaped electric pulses (rise and fall times ) with durations of 10, 40, 60, or 92 ns. The maximal amplitude of such pulses is 12.5 kV. The main advantage of this system is its ability to generate single pulses, the amplitude and duration of which can be precisely set in advance. To treat the cells, a coaxial cuvette with a 0.03-mL active volume and a 1-mm distance between the 28.3- mm2 circular-shaped electrodes was used. The system was tested on human erythrocytes. It was demonstrated that for the 92- and 40-ns duration pulse, the amplitude required to electroporate 50% of the cells was 20 and 65 kV/cm, respectively.

Published in:

Plasma Science, IEEE Transactions on  (Volume:41 ,  Issue: 10 )

Date of Publication:

Oct. 2013

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.