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In this paper, a new cross layer design of MAC and Routing protocol for OFDMA-based Multi-hop Ad-hoc Networks is proposed. In wireless network, when a route is established, the radio resource allocation problems at MAC layer may decrease the end to end performance proportionally with the length of each route. The contention at MAC layer may causes routing protocol to respond by finding new routes and routing table updates. The cross layer design allows layers to exchange state information in order to solve the problem and obtain higher performance. The proposed cross layer design of MAC and Routing protocol based the dynamic Sub-channel Assignment Algorithm in  to ensure the performance of ad-hoc and multi-hop networks is significantly improved.