High-throughput sperm sorting in a micro diffuser type fluidic system

In this study, we propose a microfluidic device with a diffuser type chamber to differentiate the sperm velocity by gradually lowering down the flow speed in diffuser by gradually increasing the width of the channel. When the sperms' moving speed match the flow speed, the sperms tend to have the ability to flow against the stream, thus separating the sperms at different specific positions along the diffuser according to their motility becomes gentle and fast. Most of the motile sperm will stay in the expanding area and the non-motile sperms will drift to outlet.