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Thermocouples fabricated out of nanowires possess a high spatial and temporal resolution. Due to their small size, nanowires exhibit different physical properties from their bulk counterparts. One of these properties, the Seebeck coefficient, specifies how well the thermocouple converts a temperature gradient into an open-circuit voltage. We have developed a characterization platform, with which the Seebeck coefficient of nanowires can be measured as required for the calibration of nanowire thermocouples and optimization of their fabrication process.