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We developed a simple method to perfuse oil-water in electrowetting displays. The theoretical model to simulate the progress of water entry into the interlayer while squeezing the oil was established. It shows that the minimum inlet pressure of water required to enter the interlayer must counteract the capillary pressure difference at the interface and the hydraulic resistance. In addition, the condition to remain the dyed oil in the pixels after the entry of water is affected by the pixel size and the height of interlayer. The perfusion experiment coincides with the simulation which confirms the theoretical conclusion that the pixel size and interlayer thickness influence the thickness of the remained oil films.