By Topic

Determination of cell concentration in 3D cell culture construct based on electrical impedance measurement by on-chip vertical electrodes

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Kin Fong Lei ; Grad. Inst. of Med. Mechatron., Chang Gung Univ., Taoyuan, Taiwan ; Che-Wei Hsu ; Shing-I Yang ; Cheng-Yuan Lin
more authors

Onsite determination of cell concentration in 3D cell culture construct is proposed based on electrical impedance measurement through on-chip vertical electrodes embedded in the 3D cell culture chamber. Cells were encapsulated in agarose gel and cultured in the 3D cell culture chamber. A pair of vertical electrodes was fabricated at the opposite sidewalls of the culture chamber for performing electrical impedance measurement. Electric field penetrated through the cells/agarose construct and the cell concentration in the construct can be estimated by the impedance change. In this study, cell concentrations of 104, 105, and 106 cells/ml can be determined directly without altering the cell property.

Published in:

Sensors, 2012 IEEE

Date of Conference:

28-31 Oct. 2012