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Hetero-Coated Magnetic Microcarriers for Point-Of-Care Diagnostics

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10 Author(s)
Palfreyman, J. ; Cavendish Lab., Univ. of Cambridge, Cambridge, UK ; Love, D. ; Philpott, A. ; Vyas, K.
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SU8 microcarriers containing magnetic thin film (40 nm cobalt) elements were fabricated by photolithography and thermal evaporation, and included a gold coating on the base. The microcarriers, which can be encoded with a magnetic barcode for later identification in a multiplexed suspension assay, now possess two distinct surfaces for further chemical derivitization. This allows us to attach different bio-molecules to each side, which we suggest, could be used to add a positive control to typical hybridization assays as a test for binding conditions, thus giving confidence against false negative events. As a proof-of-concept we developed a chemical pathway to attach 5',6'-tetramethyl rhodamine onto the gold coated side via the growth of a mixed self-assembled monolayer (characterized by Electrochemical Quartz Crystal Microbalance measurements), while also attaching fluorescein to the SU8 side after first adding a homo-bifunctional spacer to the native surface epoxide groups. Fluorescence microscopy was used to verify the success of this strategy and the feasibility of using PicoGreen as a post-hybridization reporter was studied.

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Magnetics, IEEE Transactions on  (Volume:49 ,  Issue: 1 )