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The quantification of magnetic iron oxide nanoparticles in biological systems (cells, tissues and organs) is of vital importance in the development of novel biomedical applications such as magnetofection, drug targeting or hyperthermia. Among several techniques established to detect iron in tissue, the recently developed technique of magnetic particle spectroscopy (MPS) provides signals that are specific for magnetic nanoparticles. MPS utilizes the non-linear response of an MNP sample to a strong sinusoidal excitation field of up to 25 mT amplitude and 25 kHz. We demonstrate the feasibility of this technique to quantify nanoparticle uptake in cells using a commercial magnetic particle spectrometer (Bruker BioSpin).