Cart (Loading....) | Create Account
Close category search window
 

Spatial SPION Localization in Liposome Membranes

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Bonnaud, C. ; Adolphe Merkle Inst., Univ. of Fribourg, Marly, Switzerland ; Vanhecke, D. ; Demurtas, D. ; Rothen-Rutishauser, B.
more authors

Nanocarriers, including liposomes, offer great opportunities for targeted and controlled therapy. The development in this field has led to a large panel of drug delivery systems, which can be classified into 3 different nanovector generations. However, the success of such smart materials requires the control of a large variety of properties and parameters. Unfortunately, characterization at the nanoscale is often cumbersome and many methods are still being developed. Liposomes have been characterized by cryogenic electron microscopy (CryoTEM) for quite some time, also in combination with nanoparticles, in particular with superparamagnetic iron oxide nanoparticles (SPIONs) incorporated inside the liposomal membrane. CryoTEM, unlike classical TEM, maintains the native state of the liposomes. The quick freezing of the sample immobilizes particles and liposomes exactly at their position in the suspension. Therefore, localization information can be extracted from the images. However, data must be treated extremely carefully keeping in mind that 2-D projections of a 3-D object are observed. In this paper, we discuss the analysis of cryoTEM images of liposome-particle hybrids, including the estimation of the contrast transfer function (CTF) and electron dose, as well as the correct positioning of the sample holder and tomography for accurate localization.

Published in:

Magnetics, IEEE Transactions on  (Volume:49 ,  Issue: 1 )

Date of Publication:

Jan. 2013

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.