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The channel estimation techniques based on comb-type pilot arrangement, which is used in multiple input multiple output-orthogonal frequency division multiplexing (MIMO-OFDM) system, is investigated in this paper. In view of the contradiction of the time-domain least squares (LS) channel estimation bit error rate (BER) performance and the practical realization complexity, a novel optimal pilots design method for multiple input multiple output-orthogonal frequency division multiplexing (MIMO-OFDM) is proposed. By using this approach no large matrix pseudo-inverse is required, which greatly reduces the complexity of algorithms. Simulation results show that the proposed method provides better BER performance and lower complexity. And it is easy to implement in practical systems.