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In this paper, the RuO2 sensing film is fabricated by the sputtering system, and the miniature reference electrode is fabricated by the screen printed technique for integrating the RuO2 thin film in the array flexible sensor. Furthermore, the computer numerical control technique is used to fabricate the stainless model of the fluidic channel, and polydimethylsiloxane is used as the material of the fluidic channel. Finally, the array flexible sensor is integrated fluidic channel to form the potentiometric biosensor of the fluidic. The property of the fluidic channel is analyzed by differential pressure meter to measure the pressure drop and discuss the effect of related factors. In this paper, the RuO2 based pH sensor can obtain a higher sensing value at 61.3 mV/pH with super-Nernstian response. Besides, due to silver's excellent stability and conductivity, the miniature reference electrode is fabricated by screen printed technique to fix silver at the polyethylene terephthalate substrate. The sensitivities of the sensor of 61.31, 59.65, and 56.45 mV/pH with flow rates of 10, 20, and 30 ml/min, respectively, are obtained. The experimental result demonstrates that the potentiometric biosensor can be applied to detect the fluidic concentration.